The USB4000-FL is our next-generation miniature spectrometer platform developed for fluorescence spectrometers! It provides enhanced electronic characteristics for fluorescence measurements, higher optical resolution, and a 10-microsecond integration time. When retrofitted with the L4 detection focusing lens, it increases absorbance and reduces stray light. The high-sensitivity USB4000-FL has a wavelength range of 360–1000 nm and an optical resolution of 10.0 nm (FWHM). The minimum integration time of the USB4000-FL can be set to 10 µs via software to handle short fluorescence periods. The USB4000-FL's programmable processor allows you to change operating parameters in software—control the spectrometer and external excitation sources, and even acquire data from external targets. The USB4000-FL, USB4000-FL-450, and USB-FL-395 are high-sensitivity preconfigured fluorescence spectrometers designed for fluorescence sensing applications. Each fluorescence unit is set in the 360-1000 nm wavelength range, with a 200 µm entrance slit and an L4 detector condenser for enhanced light collection efficiency. USB4000-FL: does not include an excitation source. The USB4000-FL does not contain an excitation light source, allowing flexible selection from a variety of small, low-cost, modular excitation sources, such as Ocean Optics' LED light sources. The excitation source generates pulsed or continuous signals, which are conveniently transmitted to the spectrometer, optical fibers, and sampling accessories. USB4000-FL-450 & USB4000-FL-395: come with an excitation source. The USB4000-FL-450 & USB4000-FL-395 spectrometers have the same configuration as the USB4000-FL, but they additionally include a light source. The USB4000-FL-450 uses a 470 nm LED light source, and the USB4000-FL-395 uses a 395 nm LED light source. When you are equipping the fluorescence spectrometer with a probe, we recommend that the USB4000-FL-450 can use FOXY and HIOXY probes, and the USB4000-FL-395 can use the FOSPOR probe. The spectrometer can power the LED, enabling synchronization and I2C communication functions. Moreover, the light source is also connected to a temperature sensor, with onboard memory that can store temperature and oxygen content calibration coefficients.
| Parameter | Value |
| Dimensions | 89.1 mm × 63.3 mm × 34.4 mm |
| Weight | 190 g |
| Detector | Toshiba TCD1304AP Linear CCD Array |
| Design | f/4, asymmetrical crossed Czerny-Turner optical path |
| Entrance aperture [Slit] | 200 µm slit |
| Grating | #3 grating, 600 line density, 360-1100nm bandwidth, blaze wavelength 500 nm |
| Fiber connector [0.22 numerical aperture fiber] | SMA905 with 0.22 numerical aperture fiber |
| Input focal length | 42 mm |
| Output focal length | 68 mm |
| Wavelength Range | 360~1100 nm |
| Optical resolution (FWHM) | ~10.0 nm FWHM |
| Signal-to-noise ratio [Full Signal] | 300:1 (full signal) |
| A/D | 16-bit |
| Dark noise [RMS] | 50RMS counts |
| Corrected linearity | >99.8 % |
| Stray light [600nm] | <0.05 % at 600 nm |
| Stray light [435 nm] | 0.10 % at 435 nm |
| Power Consumption [5 VDC] | 250 mA @ 5 VDC |
| Data transfer speed [Full scan to memory; USB2.0] | 5 ms |
| Data transfer speed [Full scan to memory; USB1.1] | 18 ms |
| Data transfer speed [Full scan to memory; serial port] | 300 ms |
| Input/Output | 8 digital, programmable general-purpose input/output ports |
| Operating System | USB port: Windows 98/Me/2000/XP, Mac OS X and Linux Serial port: any 32-bit Windows operating system |
| Computer interface [480 Mbps (USB1.1 compatible); RS-232 (2-wire) @ 115.2 K baud] | USB 2.0 @ 480 Mbps (USB1.1 compatible); RS-232 (2-wire) @ 115.2 K baud |
| Peripheral interface | SPI (3-wire); I2C inter-integrated circuit |
| Detection Range | 360-1000nm |
| Pixel | 3648 pixels |
| Pixel size | 8 μm × 200 μm |
| Pixel well depth | 100,000 electrons |
| Sensitivity [400 nm] | 130 photons/count |
| Sensitivity [600 nm] | 60 photons/count |
| Grating Selection | Grating #3 - groove density of 600 lines per mm, wavelength range 360-1000nm, peak wavelength 500 nm |
| HC-1 grating selection | Not selectable |
| Detector focusing mirror selection | Optional, L4 |
| OFLV filter option | OFLV-200-850; OFLV-350-1000 |
| Order-sorting filter option | Longpass OF-1 filter |
| Collimating mirror Focusing mirror | Standard or SAG+UPG |
| UV-enhanced window | Optional, UV4 |
| Wavelength range [Depending on grating selection] | Based on grating selection 360-1000 nm |
| Integration time | 10 microseconds to 65 minutes |
| Dynamic range [System] | 2 x 10⁸ (system) |
| Dynamic range [Single detection] | 1300:1 single detection |
| Analog channel | None |
| Power supply rise time | <5 s |
| Automatic zeroing | Yes |
| Trigger modes | 5 modes |
| Filtering function | Yes |
| Connector | 22-pin connector |
| Optical resolution (FWHM) | 10.0 nm FWHM |
| Data transfer speed [Full scan to memory; USB2.0] | 4 ms |
| Operating System | USB port: Windows 98/Me/2000/XP, Mac OS X and Linux; any 32-bit Windows operating system with a serial port |
| Computer interface [480 Mbps (USB1.1 compatible); RS-232 (2-wire) @ 115.2 Kb] | USB 2.0 @ 480 Mbps (USB1.1 compatible); RS-232 (2-wire) @ 115.2 Kb |
| Peripheral interface | SPI (3-wire); I 2 C inter-integrated circuit |
Stronger light collection capability, less stray light 5 trigger options Response wavelength 360-1000 nm, optical resolution 10.0nm (FWHM) 10µs integration time Electronic shutter: 10µs to open the detector The integration time in our software is set by the user. Similar to a camera's shutter speed: the specified integration time is the time the detector observes incident photons. The USB4000-FL uses a 3648-pixel Toshiba TCD1304AP linear CCD detector. Because the Toshiba detector's electronic shutter can be set via software to an integration time as short as 10 microseconds, you can measure fluorescence events that occur instantaneously, like laser pulses. Programmable microcontroller The programmable microcontroller included with the USB4000-FL provides great flexibility for controlling the spectrometer and its components. Through a 22-pin connector, you can execute all operating parameters in software: control light sources, create processes, and acquire information from external devices. You have 8 user-programmable digital input/output ports to provide interfaces with other devices. Optimal performance The USB4000-FL uses Toshiba's high-performance 3648-pixel linear CCD array detector, and an optical bench small enough to fit in the palm of your hand. Its working principle is the same as other spectrometers produced by Ocean Optics; it accepts light transmitted through a single-core fiber, disperses it through a fixed grating, and delivers it to the linear CCD array detector, whose response wavelength range is 360-1000 nanometers. Plug and play The USB4000-FL connects to a PC, PLC, or other embedded controller via USB2.0 or RS-232 serial port. Each spectrometer's respective data is programmed in a memory on the USB4000. Our spectrometer control software reads these values for thermal transfer between PCs and fast parameter setup. Additional filters The USB4000-FL can be used with our LVF series linear variable filters to build a system that separates fluorescence and excitation light well. Use our LVF series linear variable low-pass filters to fine-tune your excitation source; and/or use our LVF series linear variable high-pass filters on the detection side to obtain the strongest signal with minimal overlap. The built-in holder and cuvette clamp make the filters easy to install.
Please provide your email to download the datasheet.
Guangzhou Biaoqi Optoelectronics Technology Development Co., Ltd. was founded in 2005 and is an enterprise founded by leading entrepreneurial talents in Guangzhou Development District. It is a national high-tech enterprise and a national specialized and sophisticated "little giant" enterprise focusing on spectral rapid detection system integration and instrument development. The company integrates design, R&D, manufacturing, sales, and after-sales service, with spectral rapid detection and analysis technology as its core. Through high-speed acquisition, processing, and algorithm analysis of spectral data, it provides rapid, non-destructive, online, fully automatic detection equipment and high-end quality inspection and analysis solutions for various industries. Biaoqi Optoelectronics has established the Guangdong Provincial Engineering Technology Research Center for Rapid Spectral Measurement. Its products cover many fields such as optical component inspection, semiconductors, new energy vehicles, environmental monitoring, solar photovoltaics, new materials, biology, optoelectronics, and chemicals. Relying on strong independent R&D capabilities and independent demonstrative diversified operational advantages, Biaoqi Optoelectronics has achieved rapid development in recent years and is a leader in the domestic spectral rapid detection application industry. It has obtained numerous qualifications and honors, including national specialized and sophisticated "little giant" enterprise, national high-tech enterprise, Guangdong Province specialized and sophisticated enterprise, Guangdong Province intellectual property demonstration enterprise, and Guangzhou Development District gazelle enterprise.
Under the guidance of its core corporate values of "prudent thinking, earnest practice, innovation, and win-win", Biaoqi Optoelectronics continuously accumulates experience, builds a domestic independent brand, and customizes spectral detection application solutions for customers, developing spectral rapid detection and analysis instruments suitable for different application fields. These include the optical component inspection industry: optical component spectral sorting instruments, reflectance spectral analyzers, transmittance spectral analyzers, etc.; the jewelry identification industry: UV-visible spectral analyzers (jewelry detectors), jewelry luminescence imaging analyzers, etc.; and the scientific research field: quantum efficiency spectral analysis systems, portable Raman spectral analyzers, photoluminescence spectral analyzers, etc. Biaoqi Optoelectronics' non-destructive rapid spectral analyzers have significant advantages, featuring high efficiency, speed, and accuracy, which can help users greatly improve detection efficiency and assist the high-quality development of application industries.
At the same time, Biaoqi Optoelectronics is the South China general agent for Ocean Optics, the inventor of micro fiber optic spectrometers, an internationally renowned enterprise.
With the rapid development of science and technology, Biaoqi Optoelectronics keeps pace with technological progress and the development of the times, forging ahead on the road of rapid spectral detection, striving for perfection.