FL475FF Bandpass Fluorescence Excitation Block
Full product name: Fluorescence microscopy Mirror Unit, bandpass fluorescence excitation block. Optical configuration: excitation filter EX475/40 + dichroic beamsplitter DM500 + emission filter EM530/50. Standard accessory: dedicated protective storage case (including case). Compatible fluorescence systems: GFP, FITC, Cy2, AlexaFluor488, BODIPY 492/515, Calcein and Evans Blue.
Specification Snapshot
EX475/40 Excitation Filter - Center Wavelength
475nm
EX475/40 Excitation Filter - Half Bandwidth
40nm
EX475/40 Excitation Filter - Effective Wavelength Band [Blue Broadband Excitation]
455nm ~ 495nm
DM500 Dichroic Beamsplitter - Cutoff Wavelength
500nm
EM530/50 Emission Filter - Center Wavelength
530nm
EM530/50 Emission Filter - Half Bandwidth
50nm
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Overview
Full product name: Fluorescence microscopy Mirror Unit, bandpass fluorescence excitation block. Optical configuration: excitation filter EX475/40 + dichroic beamsplitter DM500 + emission filter EM530/50. Standard accessory: dedicated protective storage case (including case). Compatible fluorescence systems: GFP, FITC, Cy2, AlexaFluor488, BODIPY 492/515, Calcein and Evans Blue.
Specifications
| Parameter | Value |
|---|---|
| EX475/40 Excitation Filter - Center Wavelength | 475nm |
| EX475/40 Excitation Filter - Half Bandwidth | 40nm |
| EX475/40 Excitation Filter - Effective Wavelength Band [Blue Broadband Excitation] | 455nm ~ 495nm |
| DM500 Dichroic Beamsplitter - Cutoff Wavelength | 500nm |
| EM530/50 Emission Filter - Center Wavelength | 530nm |
| EM530/50 Emission Filter - Half Bandwidth | 50nm |
| EM530/50 Emission Filter - Effective Wavelength Band [Green Fluorescence Reception] | 505nm ~ 555nm |
| Compatible Fluorescent Labels - Excitation Peak [GFP] | 475 nm |
| Compatible Fluorescent Labels - Emission Peak [GFP] | 530 nm |
| Compatible Fluorescent Labels - Main Applications [GFP] | Green Fluorescent Protein Tracing |
| Compatible Fluorescent Labels - Excitation Peak [FITC] | 475 nm |
| Compatible Fluorescent Labels - Emission Peak [FITC] | 525 nm |
| Compatible Fluorescent Labels - Main Applications [FITC] | Immunofluorescence Labeling |
| Compatible Fluorescent Labels - Excitation Peak [Cy2] | 475 nm |
| Compatible Fluorescent Labels - Emission Peak [Cy2] | 520 nm |
| Compatible Fluorescent Labels - Main Applications [Cy2] | Flow Cytometry / Immunofluorescence |
| Compatible Fluorescent Labels - Excitation Peak [AlexaFluor488] | 480 nm |
| Compatible Fluorescent Labels - Emission Peak [AlexaFluor488] | 525 nm |
| Compatible Fluorescent Labels - Main Applications [AlexaFluor488] | High-Brightness Immunofluorescence Labeling |
| Compatible Fluorescent Labels - Excitation Peak [BODIPY 492/515] | 492 nm |
| Compatible Fluorescent Labels - Emission Peak [BODIPY 492/515] | 515 nm |
| Compatible Fluorescent Labels - Main Applications [BODIPY 492/515] | Lipid / Protein Fluorescent Labeling |
| Compatible Fluorescent Labels - Excitation Peak [Calcein] | 475 nm |
| Compatible Fluorescent Labels - Emission Peak [Calcein] | 530 nm |
| Compatible Fluorescent Labels - Main Applications [Calcein] | Live-Cell Calcium Imaging |
| Compatible Fluorescent Labels - Excitation Peak [Evans Blue] | 470 nm |
| Compatible Fluorescent Labels - Emission Peak [Evans Blue] | 535 nm |
| Compatible Fluorescent Labels - Main Applications [Evans Blue] | Vascular / Cell Membrane Permeability Detection |
Applications
Cell Fluorescence Imaging; Green Fluorescent Protein Observation; Immunofluorescence Staining; Calcium-Ion Imaging; Live-Cell Tracking; Histopathological Fluorescence Analysis; Microorganism Detection
Key Features
Broad Blue Excitation for Higher Efficiency,Matched GFP,FITC,AlexaFluor488 Green Dyes/Proteins;Hard Multilayers,High Transmission/Deep Blocking for Low Background/High SNR;Standard Cube Fits Most Upright/Inverted Microscopes,Plug-and-Play;Protective Dust/Moisture Case;Broad Emission Window Collects More Weak Fluorescence;DM500 Reflects Excitation/Transmits Fluorescence,Rejects Stray Light/Crosstalk
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Supplier Information
Jiangsu Situoli Instrument Co., Ltd., established in 2014, is a national high-tech enterprise focusing on the field of microscopy. It integrates R&D, manufacturing, sales, and service, providing OEM/ODM customization for complete microscopes and accessories. With standardized workshops and advanced production/inspection equipment, it has passed ISO quality management system certification. Its main products include biological, metallographic, upright, inverted, and stereo microscopes, covering objective magnifications from 2X to 100X, widely used in scientific research, industrial inspection, life sciences, etc. It has obtained over 50 invention patents and independently developed modular optical accessories such as electric fluorescence systems and imaging systems. The company is committed to becoming a leading brand in microscopic optical instruments.
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