Fluorescence Filter
Fluorescence filters extract fluorescence energy or images in fluorescence systems. Strong stray-light isolation and low autofluorescence suit PCR, fluorescence immunoassay and microscopy. Blocking typically exceeds OD5, optical density OD=-lgT. As key biomedical/life-science components, they offer deep blocking, low autofluorescence, good transmission and surface form. High-quality versions use one colorless transparent glass substrate coated on both sides, improving imaging over bonded multilayers or colored-glass-assisted blocking, though harder manufacture raises cost. A typical set comprises excitation filter, emission filter and dichroic mirror; some systems omit the dichroic, and laser excitation may eliminate the excitation filter. Excitation filter: Passes only excitation wavelengths; bandpass has largely replaced shortpass. An engraved arrow indicates recommended light direction. Emission filter: Passes sample fluorescence and blocks other wavelengths. Emission is longer/redder than excitation; bandpass or longpass types are used, with direction arrows. Dichroic mirror/beamsplitter, also called a color separator, is mounted45° to the microscope path. It reflects excitation and transmits emission, with reflectance>90% and transmission>90%. Rejected bands reflect rather than absorb; transmitted/reflected colors are complementary.
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Overview
Specifications
| Parameter | Value |
|---|---|
| Temperature | >90% |
| Closed [200-1200nm] | OD6 200-1200nm |
| Flatness [@633nm] | 1/4 lambda@633nm |
Datasheet
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