Fluorescence Bandpass Filters
Fluorescence bandpass filter is a special optical filter mainly used in fluorescence detection system (such as fluorescence microscope, flow cytometer, biochemical analyzer, etc.), whose core function is to selectively transmit the fluorescence signal of a specific band while blocking the excitation light and other stray light, in order to improve the signal-to-noise ratio and detection sensitivity. What are the optical properties of fluorescence bandpass filters? Center Wavelength (CWL): the wavelength at which the filter has the highest transmittance, which needs to match the emission peak of the fluorescent dye (e.g. FITC: ~525 nm, DAPI: ~460 nm). Full Width at Half Maximum (FWHM): the wavelength range in which the transmittance is ≥50%, commonly narrowband (10~40 nm) or broadband (50~100 nm). Peak Transmittance (Tpeak): usually >90% (up to 95% or more for high quality filters). Blocking Range: optical density (OD) in the non-passband region (e.g., excitation band) should be ≥ OD4 (i.e., transmittance < 0.01%). Parameter / Typical Value / Influence CWL (Typical Value: Selection based on fluorescent dye (e.g. Cy5: ~670 nm)): Influence: It must match the fluorescence emission peak or the signal is lost. FWHM (Typical Value: 10~40nm (narrow band)): Influence: The narrower the bandwidth, the higher the signal-to-noise ratio, but the signal strength may be reduced. Cut-off Range (Typical Value: OD4~OD6 (excitation light band)): Influence: Ensure complete blocking of excitation light (e.g., 488 nm laser) to avoid background interference. AOI (Typical Value: 0° (vertical incidence optimal)): Influence: Tilted incidence may result in a CWL offset (requires selection of an angle-compensated design).
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Overview
Specifications
| Parameter | Value |
|---|---|
| Center Wavelength (CWL) | Selection based on fluorescent dye (e.g. Cy5: ~670 nm) |
| Full Width at Half Maximum (FWHM) | 10~40nm (narrow band) |
| Cut-off Range | OD4~OD6 (excitation light band) |
| AOI | 0° (vertical incidence optimal) |
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