Quantitative Fluorescence PCR Optical Filter
Real-time fluorescent Quantities PCR(Quantitative Real-time PCR) is Reaction in the polymeric chain. (PCR) Add fluorescent Dye Or a fluorescent probe. , Use fluorescent signals to accumulate real time detection of the whole PCR Instruments for the response process 。 It's widely used in molecular diagnostics. , Molecular biology research , Areas such as phytosanitary and phytosanitary and food safety testing 。 The rationale. is Laser or LED When the light comes out of the light Different designs stimulate interference. Optical Filter Back , It's a reaction cup and a sparkling fluorescent group. , The fluorescent from the fluorescent group is interferogenic through a matching design. Optical Filter And then they were picked up by a photo-detector. 。 Collect data once in each reaction cycle , By expanding the curve in real time , Accurately CT Value , Thus Based on the Established Standard Curve Use CT Value established DNA Number of initial copies , It's a real thing. DNA Quantification 。PCR So cleverly expands the accounting and cross-cutting. , Combining spectroscopy analysis and real-time detection techniques , Use the fluorescent signal to detect it. PCR Products 。 Solutions : Common human medical equipment | Standard Seven Channels of Fluorescent Optical Filter Remarks : It's a match. Dichroic Mirror 、 Lens Group Apply Optical Component : Narrowband Filter 、 Two. Beamsplitter 、 Lens 。
Overview
Specifications
| Parameter | Value |
|---|---|
| Common medical care equipment | Use Wavelength | 350nm-800nm |
| Ex [Atto425] | 430 |
| Em [Atto425] | 480 |
| Ex [FAM] | 470 |
| Em [FAM] | 515 |
| Ex [HEX] | 535 |
| Em [HEX] | 565 |
| Ex [ROX] | 585 |
| Em [ROX] | 614 |
| Ex [CY5] | 628 |
| Em [CY5] | 670 |
| Ex [CY5.5] | 682 |
| Em [CY5.5] | 725 |
| Ex [CY7] | 750 |
| Em [CY7] | 800 |
| Ex [R365/T610] | 365 |
| Em [R365/T610] | 610 |
| Ex [R470/T525] | 470 |
| Em [R470/T525] | 525 |
| Ex [R520/T570] | 520 |
| Em [R520/T570] | 570 |
| Material | Optical Glass |
Datasheet
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Database Platform