Optical Filters for Fluorescence Microscopy
Fluorescence-microscopy filters provide high contrast and specificity by separating excitation light from fluorescence and rejecting stray light. Excitation filters are usually bandpass filters that transmit a specified short-wavelength excitation band, such as approximately 490nm for FITC. Emission filters are typically long-pass or bandpass filters that transmit longer-wavelength fluorescence, such as approximately 520nm emission from FITC, while blocking unabsorbed excitation light. A dichroic beamsplitter between the excitation and fluorescence paths reflects short-wavelength excitation toward the sample and transmits longer-wavelength fluorescence to the detector, separating the optical paths.
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Overview
Specifications
| Parameter | Value |
|---|---|
| In-Band Transmittance | ≥90% |
| Blocking Depth [Blocking Band (e.g. Excitation-Light Interference in the Emission Path)] | ≥OD6 |
Applications
Datasheet
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