QE65000-FL fluorescence spectrometer
QE65000 spectrometer is a new research-grade spectrometer combining detector, optical bench, and electronics technologies with extremely high sensitivity. It can be applied to demanding conditions of extremely low light levels, such as fluorescence, DNA sequencing, astronomy, and Raman spectroscopy. The quantum efficiency of the QE65000 research-grade spectrometer can reach 90%, and it has a high signal-to-noise ratio and fast signal processing capability. The QE65000-FL scientific-grade spectrometer for fluorescence measurement is a spectrometer preconfigured specifically for fluorescence measurement.；QE65000-FL Fluorescence Spectrometer - Product Features；•Quantum efficiency up to 90%；*Portable；QE65000-FL Fluorescence Spectrometer Technical Parameters；Physical；Detector:；Hamamatsu S7031-1006；Pixels:；1024 x 58 (1044 x 64 total pixels)；Pixel size:；24.576 μm^2；Pixel well depth:；1000 Ke-；Dark current:；4000 e-/pixel/sec @ 25 �C; 200 e-/pixel/sec @ 0 �C
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Overview
Specifications
| Parameter | Value |
|---|---|
| Dimensions [Physical] | 182 mm × 110 mm × 47 mm |
| Weight [Physical] | 1.18 kg (without power supply) |
| Detector | Hamamatsu S7031-1006 |
| Detector range | 200-1100 nm |
| Pixels [Detector] | 1024 x 58 (1044 x 64 total pixels) |
| Pixel size [Detector] | 24.576 μm² |
| Pixel well depth [Detector] | 1000 Ke- |
| Sensitivity [Detector] | ~0.065 counts / e- |
| Quantum efficiency [Detector] | 90 % peak; 65 % at 250 nm |
| Design [Optical Bench] | f/4, Symmetrical crossed Czerny-Turner |
| Focal length [Optical Bench] | 101.6 mm input and output |
| Entrance aperture [Optical Bench] | 200 μm wide slit |
| Grating [Optical Bench] | HC1-QE |
| OFLV filter [Optical Bench] | OFLV-QE-350 (350-1100 nm) |
| Collimating and focusing mirrors [Optical Bench] | Standard only |
| UV enhanced window [Optical Bench] | No |
| Fiber optic connector [Optical Bench] | SMA 905 to 0.22 numerical aperture single-strand optical fiber |
| Wavelength range [Spectroscopic] | 350-1100 nm (grating dependent) |
| Optical resolution [Spectroscopic] | ~6 nm FWHM |
| Signal-to-noise ratio [Spectroscopic] | 1000:1 (at full signal) |
| A/D resolution [Spectroscopic] | 16 bit |
| Dark noise [Spectroscopic] | 3 RMS counts |
| Dynamic range [Spectroscopic] | 7.5 x 10⁹ (system), 25000:1 for a single acquisition |
| Integration time [Spectroscopic] | 8 ms to 15 minutes |
| Stray light [Spectroscopic] | <0.08 % at 600 nm; 0.4 % at 435 nm |
| Corrected linearity [Spectroscopic] | >99.8 % |
| Power consumption [Electronics；5 VDC (no TE cooling)；3.5 A @ 5 VDC (with TE cooling)] | 500 mA @ 5 VDC (no TE cooling); 3.5 A @ 5 VDC (with TE cooling) |
| Data transfer speed [Electronics] | Full scans to memory every 7 ms with USB 2.0 port, 18 ms with USB1.1 port, 300 ms with serial port |
| Inputs/Outputs [Electronics] | 10 onboard digital user-programmable GPIOs (general purpose inputs/outputs) |
| Analog channels [Electronics] | No |
| Auto nulling [Electronics] | Yes |
| Breakout box compatibility [Electronics] | Yes |
| Trigger modes [Electronics] | 4 modes |
| Strobe functions [Electronics] | No |
| Gated delay feature [Electronics] | Yes |
| Connector [Electronics] | 30-pin connector |
| Power-up time [Electronics] | <5 seconds |
| Dark current [Electronics；25 °C；200 e-/pixel/sec @ 0 °C] | 4000 e-/pixel/sec @ 25 °C; 200 e-/pixel/sec @ 0 °C |
| Operating systems [Computer] | Windows 98/Me/2000/XP, Mac OS X and Linux with USB port; Any 32-bit Windows OS with serial port |
| Computer interfaces [Computer；480 Mbps；RS-232 (2-wire) @ 115.2 K baud] | USB 2.0 @ 480 Mbps; RS-232 (2-wire) @ 115.2 K baud |
| Peripheral interfaces [Computer] | SPI (3-wire); I2C inter-integrated circuit |
| Temperature limits [Computer] | 0 °C to 50.0 °C; no condensation |
| Set point [Computer] | Software controlled; lowest set point is 40 °C below ambient |
| Stability [Computer] | +/-0.1 °C of set temperature in <2 minutes |
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